How Did I get here?

As I prepared for my qualifying exam, my P.I. (principal investigator, AKA my boss) told me that he wanted me to begin some experiments with a lab that was on the opposite coast. I was not too keen on the idea as this would mean traveling a great deal prior to my exam and I wanted as much time as possible to prepare. Prior to my exam I had 2 trips planned, each a total of 3 days at a time, taking roughly 9 hours for travel (including layovers and whatnot) each direction. While the turnaround time was short, it left me exhausted and in a perpetual state of panic trying to make a good impression but also trying to get as much knowledge crammed into my brain.
My exam came and went, leaving me with this semi anticlimactic feeling, and I began working on even more projects in the lab than I had before. It was soon time for me to travel again to the other coast but this time for 10 days. As of right now, I'm 7 days in. It has been, a whirlwind of events and I feel almost like I live here now, which is odd. It is surprising how rapidly we adjust to our situations, we adapt much quicker and things become much more commonplace than what I would expect. Everyone from the lab that I am visiting has been both kind and supportive. All of the students, the post-docs, the faculty, all amazing. I feel very at home with them all, they've made me feel incredibly welcome. That doesn't detract from my longing for my home though, regardless of the efforts made by them, I still pining for my fiance, I miss my friends, my family, and my pets. I miss my bed. I miss the freedom of my own kitchen. There are so many things we don't think about and take for granted, and they are as simple as just transporting yourself too and from work....shit I miss my car.
Longing aside, I do find it strange that I find myself on the opposite side of the continent, doing science in a different lab and learning things I never thought I would be doing. In my head, I still see myself as a kid, when I look in the mirror, I see a scared teenager who isn't quite sure what they're doing, but here I am 28 (almost 29 in a few months) and I'm being paid to learn new techniques, to travel, to meet people, and to see the world.
Science has given me a lot and I don't know how I got here. I know I work hard, but so many people do, and don't get the same opportunities. Maybe it is luck, maybe it is something I did or didn't do. I'm not trying to humble brag but it is a weird thing that I'm still coming to terms with. I know that this isn't celebrity, most people in science won't achieve fame or world wide recognition. That being said there are times where I think it is important to take a step back and look at your life and appreciate the opportunities that have been gifted to us. Life is weird, it isn't fair, and it isn't a game that we should let ourselves be observers of. It is a full contact sport and if you don't play hard, you'll get body-checked and be out for a long time. I like taking a timeout to see what the score is, grab some water, catch my breath, and get back in the game. This is one of those moments where I'm looking at the time on the clock, looking at the score, and wondering how things worked out this way. I'm not leading by much, but I have to say, I'm not doing poorly either. There are a lot of teammates to thank (my parents, my fiance, my teachers, my friends) but we aren't even at half time yet. So it is time to dig in and push this thing up another notch.
All in all, it is really cool that I get to be here, doing these things, and I kind of feel like a kid who watched sports from the sidelines...only to have the pros call me onto the field and tell me to play with them. I feel like any second now I'm either going to get hit hard by one of the pros or wake up and find this is all a dream. I guess I'll just have to wait and see...in the meantime, I guess it's time to play ball.

I meant to keep updating, I really did.

I've meant to keep updating this thing, I swear I did. I promised myself that I would spend 1 hour a week updating this blog, I mean that isn't that much time, right? I also swore to keep running, at least 5 days a week for 30 minutes - 1 hour. Not that much of a time commitment to myself, after all, I deserve some me time, right? I was wrong. I was gravely mistaken.

So, not to make excuses, but let me just review the goings on of the last year.
I took the remaining classes necessary for me to be able to sit for my qualifying exam. I bumped up production of my own project dramatically, took on a back up project and when our lab manager left, I began to assume her duties. Those duties are not trivial ones either and do place a high demand on my time. So when I wasn't in the lab, I was at home studying or reading for class.
In this last year, I also presented a poster in NOLA which was great, my first real poster session since my masters with all the people in the new field I've chosen. As the spring quarter began, I knew that the QE season was upon us. I began trying to get the members of my cohort together to study on a weekly basis, which started out well but quickly petered out. Also, I was asked to take on a collaboration with a lab located on the opposite coast, which meant that I would have to fly to and from on a semi-regular basis to actually do the experiments myself in their facility. While I saw this as an awesome opportunity, I also saw the time commitment it was going to take and I was already concerned about my QE. I voiced my concerns to my boss but he seemed little interested and pushed on. I made 2 trips out to the other coast, each trip lasting a total of 3 days (including travel time) and all requiring layovers. These 2 trips ended up being 8 and 4 weeks before my QE. Needless to say, while I was flying I was also reading and reviewing. In the end, my boss also gave me 3 weeks off to study for the exam and review, I feel like I could have used more time but, it was sufficient.

I would also like to paint a picture of my QE since everyone's is different, and the format itself depends upon the university as well as the department. Mine was a 3 hour exam, split into 2 parts. Part 1 being focused solely on my project, I was supposed to get 20 minutes of uninterrupted time to explain my project's hypothesis, aims, and hypothetical outcomes. After which 5 professors try to poke holes in the project and see how well you can come up with answers based on your knowledge and your background. We then get a 10 minute break, followed by Part 2, which is focused on examining general knowledge of the topic, 2 inside areas, 1 outside area and a designated emphasis (equivalent to a minor as an undergrad). Each of these areas are represented by a professor on your committee and they get to ask you questions until they are satisfied of your knowledge. At the end, they kick you out of the room and deliberate if you demonstrated the knowledge base required to advance towards candidacy. If you don't, you can retake the exam 1 time, if you should not pass, there is no option to "Master out", the university requires removal from the program. So no pressure not pass, right?
From my own experience, I did not feel prepared going into the exam. I knew how much I didn't know and that was terrifying. I knew my project well, but you never know the kinds of questions that will be brought up and what kind of perspective people will bring to the table. I walked out of my exam with my head hanging low, expecting that I had failed. Given how hard the questions were, how persistent they hammered on some topics, I was sure I would have to retake the exam. I began to ask myself what I was doing, why I was even in the program. Was it all a mistake?
Luckily, and I'm sure by the skin of my teeth, I passed. My 10 minute existential crises was for nothing, but it is surprising what those 10 minutes can do to you.
I spent the weekend relaxing and taking time to just...exist without feeling rushed to do something. It was a great 3 day weekend.

My preparation for the QE was exhaustive, the number of papers I read for my topic insane. I also chose to do my outside area on a topic that I actually hadn't taken as a course, so I had 10 weeks of material to learn on my own in a few days worth of time really. The review group that I had set up only made it through a little more than half of the textbook and there were still other areas that needed to be studied. I could feel knowledge oozing from my brain and I felt like my retention rate was diminishing. I'm sure that in the process, in order to make more room, I had to delete memories of my childhood or important information linked to something somewhere...though what it may be, eludes me... as I would expect it would.

As I began back in the lab, I was excited to get started. During all of this QE nonsense, I agreed to take on an undergraduate trainee, take on another collaboration with our department chair's lab, and write a mini review for a journal. I met with my boss yesterday and he put me on another project. So all told, I have about 4 projects to work on and 1 paper to write. Oh, and I had a recent methods paper accepted where I am second author. So...the stress level comes back up to 11 and we're off to the races again.

I have friends visiting this weekend, I fly out on Monday to visit the collaborators, fly back Tuesday afternoon. I have a massive experiment that will span Thursday-Friday and then I catch a flight to go visit my family for the weekend to celebrate my dad's birthday. The pressure won't stop. So much to get done in so little time on so little sleep.

Also, some happy news, I also got engaged last February, marking 5 years together, and I'm sure we'll begin planning the actual wedding soon enough.

"I Open at the Close"

When I last updated this blog, I was about to leave for Europe for three weeks. It has now been 2 months since my last posting. I had thought about posting, so many times, but the words weren't coming and I always figured I'd find my words tomorrow. A dangerous habit to get into. "Tomorrow" is a dangerous word and I don't like using it. 

So...no longer tomorrow...Today. Now.

Lets begin with my trip through Europe, and then I'll get to the lab stuff 

I've never traveled those distances before, it was nerve wracking to say the least. My flight wasn't so bad; everywhere I landed, they spoke English, which was calming. I was worried I'd have to translate something or figure out what was happening. I had a wonderful experience when I flew on Lufthansa; I highly recommend flying with them given the opportunity. When I landed in Pisa, I had to wait at the airport for 2 hours, till representatives from the conference arrived with the bus. I arrived at the hotel, which was beautiful; it was off, deep in the countryside, and had a view of the valley below. I didn't have much time, so I took a quick shower and headed off to dinner. The meal was delicious (and not just because I had been living on airline food for the past 17+hrs). Immediately following we had the keynote speaker addressing the group; this would have been wonderful had not everyone there been falling asleep from exhaustion. Though I had no trouble sleeping the first night, I did the subsequent 3, waking up at 3-4 in the morning local time, unable to go back to sleep; this was the only part of the conference I did not enjoy. In the week I was at this conference, I met wonderful scientists. I got to hear some great talks, see some interesting science and go on some beautiful walks through the country. Each meal was just as spectacular as the first and in the end, I fell in love with Italy. The week ended with a wine-tasting at a local vineyard and a banquet back at the hotel. That night, one of the most prominent members in the DNA repair field asked to sit next to me at dinner; him and I had a wonderful conversation. It is astonishing to see the how humble this brilliant mind can be; to know the kind of work he does and to still stay so remarkably grounded is something I could only ever wish to obtain. 

At the end of the conference, I headed back to the Pisa airport where I waited for several hours for my girlfriend to arrive. Once she landed, we hailed a taxi and headed for our hotel, located fairly close by. Our room wasn't quite ready, so we walked around for a bit, found some food and came back. We rested for a bit, called home and looked up the local sites. We kept the evening short because we had booked a tour that would last 13 days and we knew we would be fairly exhausted, so we did our best to get as much rest as we could. Our second day in Pisa, we decided to go for a walk and found our way, quite accidentally I might add, at the leaning tower. We walked around a bit, took some photos (no, none of the touristy ones of us supporting the tower) and found a Hop on - Hop off tour bus that we had booked weeks before. This red double-decker bus was a great idea; we hopped on and took the tour a few times before we had decided we had had enough and would like to have lunch. Luckily for us, our tour bus stopped just outside our hotel (which was situated just across the street from the train station). 

The next day we took the train to Rome (and barring a slight error, which cost us an extra 45 euro) we arrived at our hotel with too much trouble. Here we met our tour director and a few of the others that would be traveling with us. From that night on, we did not stop. The trip was a wonderful blur. We saw amazing things. I won't go heavily into specifics, but I will say that in 13 days we went from Rome, to Pisa, to Florence, to Venice, to Innsbruck- Austria, to Liechtenstein, to Luzern- Switzerland, to Paris- France and then into London- England. We met 8 Aussies (4 groups of 2) that were traveling with us, they made this experience so much fun; we had several dinners with them, the conversations and comradery added to the tour. I know we wouldn't have had as wonderful a time had we not met them.

During this trip, I took a total of 2,300+ pictures and combined a good portion of them into 2 photo albums that adequately summarize my trip. They were rather costly (having them printed through iPhoto, for $125 a book) but they are well worth it. I didn't spend much on souvenirs for myself, so those took the place of what I could have bought. 

Last Days in the Lab 

I returned, after the trip, for about 2 months of lab work. I had signed on, 3 years ago, to do some collaborative work with another, more prestigious, university. The initial proposal of work was modest and certainly accomplishable in a short amount of time; however, after they got the first glimpses of the data, they wanted to change the parameters. This required much more time and so, off and on throughout my Master's degree, I have been working on this side project simultaneously. In these last 2 months I spent 10-14 hour days, 6 days a week in a room just large enough to fit a large microscope and 2 computers. I sat and analyzed microscopy images, in the dark for that time. That process begins to take a toll on a person and can leave you grumpy, disgruntled and disjointed. I also saw my time in the lab winding down and I knew that it was also starting to take its toll.

Several weeks ago, two new undergraduate students joined my lab to finish some of the main work I had done as a masters student. This meant preparing things for them and showing them where everything was. I began to feel myself being separated from the things I had invested so much time in; I even felts a bit of resentment towards these new students. I mean, who could love this projects like I did? Who would dedicate themselves, bleed for this lab like I have? None of them would know the struggles of the project, the frustrations, and the accomplishments. I didn't hold that against them, but I was certainly sad to see my project change hands. 

Last Friday I pulled my last all nighter in the lab. 20 hours in that tiny dark room analysing data. I finished at 5 in the morning and walked out, feeling exhausted and freed. There was something that wasn't quite final though. I still had to clean out my space in the lab and tabulate the data. Yesterday I did just that. I began saying my goodbyes to the professors on campus and all the support staff. They've all said that I would be missed, that I had changed the department and how much of an impact I had had on my PI's life. None of that really hit...none of it...until today, when I had packed up the things in my desk, walked to my PI's office, and handed her my keys. She took them and hugged me...tightly for what seemed like forever. I began to tear up, my heart was and is breaking; I now leave behind a home. It will never be the same and I will forever miss being in that lab. I've grown up a lot in these last 6 years; I've traveled the world, I've loved, I've lost, I've studied, I've partied and I've grown. My PI and I have grown close, she has been more than a mentor, but a friend.

I've now left one "home". In another month, I leave my home for grad school. 

I know that, in a very short amount of time, I will return to both places and things will have changed. Both will always be home, but they will no longer be my home and that thought...it breaks my heart even more. I know moving on is good, and it's necessary, but it is hard and I am falling apart. 

So...I'll do my best to smile, say "thank you for the memories" and get excited about what is to come.

A sigh of relief

The culmination of a 3 year body of work has been defended.
I can exhale now and smile as this huge weight has finally been lifted.

In the hours before my defense (yesterday 4/29/13 at 3:30pm) I was frantically reviewing my notes, practicing slides and trying my best to anticipate all of the questions that may be asked. Though my actual talk only consisted of 38 slides in total (taking 47 minutes to present) I had an additional 40 slides that could have been utilized had anyone asked some difficult questions. I had everything I could imagine listed there and was terrified that a hole in my knowledge would be exposed to the rest of the chemistry department; terrified that they would see, in this last stage of obtaining my master's, that I didn't really belong here.

The room slowly filled with family and friends as the hour drew nearer. I was nervous and excited. It was wonderful to see old friends, a former teacher from my high school and all of the other grad students who came to watch. The faculty slowly filed in, it seemed. The room was packed; normally, during a talk, a room may be 1/4 - 1/2 full. There was standing room only as the time drew nearer.

A professor introduced me and I began speaking. At first, the nervousness was almost unbearable, I was having a hard time remembering what I wanted to say and the way I wanted to say it. As I continued though the nerves subsided. Eventually it was, for the most part, exactly as I had practiced. I stumbled a few times in my speaking, but nothing that was too detrimental. At times I tuned in and out of my own speech. I was on "autopilot" speaking the way I had practiced it many times before alone in my room. About half way through I realized that this all was really happening, that I was in the middle of my defense and it was impressive how real and distant it all felt at the same time.

I looked out into the audience often and saw the faces of those that love me smiling back. It was comforting to see that they were there and happy to see my work. I also saw some very confused faces and it made me think to myself "Oh crap, I've lost these people, they're not following the logic." In truth, you do lose some of your audience as you talk, and I had figured I would. My thesis is a complex story with very difficult logic to follow; as my professor calls it "mental gymnastics."

When I concluded, I thanked the audience and opened the floor for questions. I was asked a few, but none that required the slides I had prepared. They were explanation questions of the slides I had presented; i.e. people wanted another look at a graph or didactic I had provided. Nothing was particularly challenging (with the exception of one theoretical question). I was slightly disappointed that I didn't get to show off the slides I had prepared but was thankful for the cursory level of questioning.

The audience slowly filed out of the room, most stopping to shake my hand and congratulate me. It was definitely a great moment. The only people that stayed behind were my committee members and, once the room was empty, reviewed some changes that needed to be made to my final draft of my thesis. There were no major suggestions or revisions, just some typographical things that needed changing...potentially a change in headings here or there.

Once this committee meeting had concluded we returned to my lab where we celebrated my defense. My professor has a 2 part ritual for such events; a speech and the popping of a cork. My professor gave one of the most touching and heartfelt speeches I've ever seen. She spoke about how long we've known each other, the level of dedication I have, and the person I've become. She began to tear up while talking; I fought back tears and so did she. I've spent 8 years on this campus, 6 of which have been in her lab. I was in one of the first classes she ever taught. Her and I have been through a lot together and it will be tough, for the both of us, to not see each other or work with one another. I'm going to miss this place.  At the conclusion of her speech, I hugged her tightly and said thank you. She then shook a bottle of champaign and I popped the cork in the lab ceiling; I then circled the area, signed and dated it. This honor is reserved only for grad students in the lab. It is a physical representation of the "mark" we've left on the lab.

After which we all talked and celebrated. Elation isn't a strong enough word.

As the after party died down, I changed into some street clothing and my girlfriend, parents and I headed to a baseball game to celebrate. Though the team didn't win, we still had a great time together. It was an amazing night.

I fell asleep last night faster than I have in a long time and awoke with a smile. Something I've not done in years. Now, I sit and make the revisions. I'm one step closer to the final version. One step closer to being done for good. I'm a Master's student...about to become a PhD student...

It is all so close and all so exciting